組學與大數據研究中心

胥國勇
時間:2020-12-08 02:05:11點擊量:

                               

                                      

教授、博士生導師



Guoyong Xu (Ph.D., Professor)

Email

guoyong.xu@whu.edu.cn

Lab Page

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu

http://uorflight.whu.edu.cn/


Education

Sep 2007 – Jul 2012   Tsinghua University, China Ph.D.

Sep 2003 – Jul 2007   Nankai University, China B.S.


Working Experience

Jan 2018 – Now    Wuhan University, China Professor

Aug 2013 – Dec 2017   Duke University, U.S.A.  Postdoc

Jul 2012 – Jul 2013    Tsinghua University, China Postdoc

Oct 2010 – Jul 2013   Tsinghua University, China College Counselor


Research Interests:

Our Biotechnology & Interdisciplinary Research Lab is aiming to use interdisciplinary strategy to study ‘Translational Control’, ‘Plant Immunity’ and ‘Precision Agriculture’.

We are focusing on one fundamental mechanism, translational control. In the past 20 years, the community of plant defense have had a clear picture of immune regulation at both transcription and post-translation levels. Our work at Duke reveals that global translational reprogramming is a fundamental layer of immune regulation in plants. Using uORF-mediated translation mechanism, we have successfully engineered disease resistant plants without fitness costs (Xu et al., nature22371, Xu et al., nature22372). (Watchhttp://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/). At Wuhan university, we have setup the following platforms: RNA-related sequencing platform including RIP-seq, RNA-seq and Ribo-seq to discover novel regulatory RNA elements; Luciferase-based genetic screening platform to find out the trans-acting factors associated with those RNA elements, such as uORF; and Microscopy-based cell biology platform to study phase separation and the spatiotemporal organization and dynamics of translation. We are aiming to illustrate the significance of translational control during plant-microbe interaction and uncover more natural and synthetic regulatory RNA elements to control mRNA translation efficiency and stability, in turn to fine tune defense gene expression for precision agriculture.

We are focusing on two basic biological questions. One is ‘Bio-triangle relationshipplant-pathogen-endophyte’. Overuse of pesticides and fertilizers and gain of resistant traits must ruin the harmonious bio-triangle relationship. We are working on the molecular mechanisms of Arabidopsis-Pseudomonas-entophytic bacteria bio-triangle interaction model to help engineer disease resistant crops. The other question is ‘Trait-triangle relationship: defense-yield-quality’. High yield usually compromises crop defense and quality. We are working on the molecular mechanisms of balancing the trait-triangle relationship to meet the increasing requirement of high-quality crops.  

To address those questions, we are actively developing novel Biotechniques with the introduction of different disciplines including omics and information technology, microfluidic technology, and synthetic chemistry and biology.  


論文* Co-first author; # corresponding

1. Ruixia Niu*, Yulu Zhou*, Yu Zhang, Rui Mou, Zhijuan Tang, Zhao Wang, Guilong Zhou, Sibin Guo, Meng Yuan, Guoyong Xu#: uORFlight: a vehicle toward uORF-mediated translational regulation mechanisms in eukaryotes. Database 03/2020, DOI: 10.1093/database/baaa007

2. Jingjing Tian, Guoyong Xu#, Meng Yuan#: Towards engineering broad-spectrum disease-resistant crops.  Trends in Plant Science 02/2020, DOI: 10.1016/j.tplants.2020.02.012 (Note: Spotlight)

3. Jingjing Tian, Guoyong Xu#, Meng Yuan#: Precise editing enables crop broad-spectrum resistance. Molecular Plant 11/2019;, DOI:10.1016/j.molp.2019.11.006 (Note: Spotlight)

4. Heejin Yoo*, George H. Greene*, Meng Yuan*, Guoyong Xu, Derek Burton, Lijing Liu, Jorge Marqués, Xinnian Dong: Translational Regulation of Metabolic Dynamics during Effector-Triggered Immunity. Molecular Plant 09/2019;, DOI:10.1016/j.molp.2019.09.009

5. Shanshan Wang*, Ke Xie*, Guoyong Xu*, Huarui Zhou, Qiang Guo, Jingyi Wu, Zengwei Liao, Na Liu, Yan Wang, Yule Liu: Plant G proteins interact with ER luminal protein receptors to regulate ER retrieval: Signaling between ERD2s and G proteins. Journal of Integrative Plant Biology 03/2018;, DOI:10.1111/jipb.12648

6. Guoyong Xu*, George H. Greene*, Heejin Yoo*, Lijing Liu, Jorge Marqués, Jonathan Motley, Xinnian Dong: Global translational reprogramming is a fundamental layer of immune regulation in plants. Nature 04/17; 545(7655): 487-490., DOI:10.1038/nature22371

7. Guoyong Xu*, Meng Yuan*, Chaoren Ai, Lijing Liu, Edward Zhuang, Sargis Karapetyan, Shiping Wang, Xinnian Dong: uORF-mediated translation allows engineered plant disease resistance without fitness cost. Nature 04/17; 545(7655): 491-494., DOI:10.1038/nature22372

8. Guoyong Xu*, Shanshan Wang*, Shaojie Han, Ke Xie, Yan Wang, Jinlin Li, Yule Liu: Plant Bax Inhibitor-1 interacts with ATG6 to regulate autophagy and programmed cell death. Autophagy 07/2017; 13(7):1161-1175., DOI: 10.1080/15548627.2017.1320633

9. Guoyong Xu, Yule Liu: Plant ERD2s self-interact and interact with GTPase-activating proteins and ADP-ribosylation factor 1. Plant signaling & behavior 09/2012; 7(9)., DOI:10.4161/psb.21217

10. Guoyong Xu*, Sizhun Li*, Ke Xie, Qiang Zhang, Yan Wang, Yang Tang, Dong Liu, Yiguo Hong, Chenyang He, Yule Liu: Plant ERD2-like proteins function as ER luminal protein receptors and participate in programmed cell death during innate immunity. The Plant Journal 05/2012; 72(1):57-69., DOI:10.1111/j.1365-313X.2012.05053.x

11. Guoyong Xu*, Ning Sui*, Yang Tang, Ke Xie, Yizhen Lai, Yule Liu: One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants. New Phytologist 07/2010; 187(1):240-50., DOI:10.1111/j.1469-8137.2010.03253.x




實驗室網頁

https://www.researchgate.net/lab/Guoyong-Xu-Lab-Translatome-Study-of-Plant-Immunity-Guoyong-Xu

http://uorflight.whu.edu.cn/

郵箱

guoyong.xu@whu.edu.cn


教育經歷

20039月 – 20077 南開大學生命科學學院 學士

20079月 – 20127 清華大學生命科學學院 博士


工作經歷

20101020137 清華大學生命科學學院 醫藥實驗班本科輔導員

20127月 – 20137 清華大學生命科學學院 博士后  

20138月 – 201712 杜克大學生物系   博士后  

20181月起    武漢大學高等研究院  教授、博士生導師



主要研究概述

  我們組建了生物技術交叉研究實驗室,利用多學科交叉融合研究:蛋白質翻譯調控(Translational Control)、植物抗病分子機制(Plant Immunity)和精準作物改良(Precision Agriculture)等。

我們主要研究一個生物學機制:蛋白質翻譯調控。在過去20多年里,我們領域對植物免疫反應的分子機制有了深入的了解,但都集中于轉錄(Transcription)和蛋白質翻譯后(Post-translation)水平上的調控,而對翻譯(mRNA translation)水平上的調控研究還處于起步階段。負責人回國前的工作揭示了蛋白質翻譯調控對植物建立免疫反應發揮了關鍵作用,并利用了上游閱讀框(uORF)介導的翻譯調控機制有效解決了農業生產中抗病性增強與產量受損的矛盾(Xu et al., nature22371Xu et al., nature22372)。可觀看(http://simplebiologist.com/2017/06/23/plant-defense-disease-resistance/)。目前,我們擬建立基于測序技術的方法,包括RIP-seqRNA-seqRibo-seq來研究新的mRNA調控元件;基于Luciferase的高通量遺傳篩選體系來研究控制uORFmRNA元件功能的基因;基于Microscopy的細胞生物學體系來研究Phase Separation現象和翻譯調控的時空關系;基于理論研究尋找天然和人工合成的翻譯調控元件控制mRNA翻譯效率和穩定性,從而調節植物抗性,實現精準作物改良。

我們關注兩個生物學問題(一)、植物、病原和內生菌之間的關系(Bio-triangleplant-pathogen-endophyte:農藥、化肥的使用以及抗病性狀的獲得極大可能破壞三者關系。我們以擬南芥-假單胞病原菌-內生細菌為模型,研究發病前后三者之間互作的分子機制,為抗病性狀的優化提供理論支撐。(二)、 作物抗性、產量和品質之間的關系(Trait-triangle: defense-yield-quality):長期對產量性狀的極致追求導致了抗性和品質性狀的丟失。理解平衡三者關系的分子機制將有助于滿足日益增長的高品質生活需求。

我們以生物技術為核心,融合三個學科交叉方向:(一)組學與信息技術;(二)微流芯片控制技術;(三)合成化學及合成生物技術,來研究上述一個機制和兩個生物學問題。


本科生、研究生、博士后:本實驗室追求知識的多樣性,熱忱歡迎具有不同背景的本科生、研究生、博士后加入。



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